mFreSR™ is a serum-free cryopreservation medium designed for the cryopreservation of human embryonic and induced pluripotent stem cells (ES and iPS cells). mFreSR™ contains DMSO and is complete and ready-to-use. Together with mTeSR™1, TeSR™2, or mTeSR™ Plus, mFreSR™ eliminates the use of feeders and serum. Human ES and iPS cells cryopreserved in mFreSR™ have thawing efficiencies higher than reported conventional thawing methods using serum.