MesenCult™-ACF Freezing Medium is a defined, serum-free, and animal component-free medium for the cryopreservation of mesenchymal stem cells (MSCs). This complete and ready-to-use medium is recommended for human MSCs previously cultured in MesenCult™-ACF Plus Medium (Catalog #05445) or MesenCult™ Medium (MesenCult™ Proliferation Kit; Catalog #05411). Frozen human MSCs should be stored at -135°C (liquid nitrogen) or colder.