FreSR™-S is a defined, serum-free, and animal component-free medium for the cryopreservation of human pluripotent stem cells (hPSCs) as single cells. This complete and ready-to-use medium is recommended for hPSCs cultured in mTeSR™1, mTeSR™ Plus, TeSR™2, or TeSR™-E8™. Cryopreserved hPSCs should be stored at -135°C (liquid nitrogen) or colder.